Supercoiled DNA/transfection

Jean-Claude irminger irminger at cmu.unige.ch
Fri Aug 15 02:39:21 EST 1997


Is it possible to transfect cells with relaxed circles or is it important to
transfect with supercoiled DNA? Is it method dependent? Is there an easy way
to fix relaxed circles to obtain supercoil DNA?
Thanks for your help



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