hot-start PCR

[bqamar at paknet1.ptc.pk]

In article <33f46af4.712157 at news.unitel.co.kr>,
  microbio at amc.ulsan.ac.kr wrote:
>
> Hot-started PCR was performed with paraffin wax bead.
> It's amplification efficiency was lower than manual hot-start PCR
> (after denaturation step, open tube cap and add Taq.pol)
> Any thoughts about this results?
> I think this result was due to Taq. pol degeneration during
> degeneration step or uncomplete melting of wax-layer..
> but I can't sure..

If you want to do hot start reactions I will recommend TaqGold from
PE/ABI the TaqStart Antibody from Clontech.  I have used both of them and
find them quite satisfactory.  Get rid of the Wax once and for all.

Raheel Qamar

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