B95-8 EBV-DNA copies

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Sun Aug 17 15:24:55 EST 1997

On Fri, 01 Aug 1997 19:53:33 GMT,
Department of Virology (virology at mbox.unipa.it) wrote:

>a friend of mine needs to know the number of copies of DNA of the
>Epstein-Barr virus in the lymphoblastoid cell line B95-8. Does anyone
>can help? 

My understanding is that it will be difficult, if not impossible
to determine the copy number of EBV genomes in B95-8 cells.  B95-8
EBV, unlike Raji EBV, is competent to undergo lytic replication,
and the virus within a few cells in each population of B95-8 cells 
does spontaneously switch from latent to lytic replication.  

As the number of cells which contain lytically replicating virus 
is unknown it is very difficult to quantitate the number of EBV
genomes per B95-8 cell.

Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu

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