Help???How to cip treatment with blunt-end vector???

Rick Davis richard..davis at
Mon Aug 18 16:46:04 EST 1997

On Sun, 17 Aug 1997 05:47:52 GMT, oravaxcm at (Charles A
Miller) wrote:

>Hiranya Roychowdhury (hroychow at NMSU.EDU) wrote:
>: At 04:48 PM 8/15/97 GMT, User wrote:
>: >Hi,
>: >Does someone know how to CIP treatment with blunt-end vector???
>: >many thanks,
>: >dong
>: >
>: >
>: Look it up in Sambrook et al. This is hardly a problem that warrants such a
>: posting on the net.
>: Dr. Hiranya Sankar Roychowdhury
>: Plant Genetic Engineering Lab.
>: New Mexico State University
>: Las Cruces, NM 88003
>: Ph. (505) 646-5785
>: hroychow at
>I know that I for one feel I should CHARGE my precious time to this person 
>for the time it took out of my day to read this post regarding 
>dephosphorylation of vector. While I am trying not to sound like too much
>of a jerk, come one...the net (FULL of prnography, blatant commercial ads, 
>and junk mail) is not holy ground in any of the religions with which I am 
>familiar. Whatever happened to the philosophy that " if you DON'T know the 
>answer, then there is no such thing as a stupid question..."? Perhaps had
>this person asked this question at a conference held by some group of
>professional molecular biologists, then maybe they would be wasting 
>someone's precious 10 seconds. If I had a question regarding an ELISA, and 
>I chose to ask someone on bionet.immunology, I would hope that I wouldn't
>be belittled for not knowing everything. Perhaps if some university
>out there would offer an all encompassing PhD, then we would all
>know everything! I really hate to post a follow-up like this to a bionet 
>group, but it really bothers me when a legitimate question (maybe not to
>everyone, but obviously to the original poster) is regarded as tripe.
>I suppose that the next time I ask a question, it ought to be something 
>that no one here could possibly answer. 
>Sorry for wasting such precious net time. I know that it could be better 
>used saving lives...(intense sarcasm)
>chuck miller
>oravaxcm at

You're quite correct, Chuck-it was a waste of time to post your
reponse, but not for the reason you cited. Dr. Puppychow's
reply was so rude and inappropriate that it did not even merit
any kind of response from any of us.

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