Help: SP6/T7 specificity

Z. Zhao zxz4 at PSU.EDU
Mon Aug 18 09:37:29 EST 1997


I would believe that you did not use an enzyme to cut the plasmid near or 
within the T7 promoter that could give you some bases for T7 polymerase 
to sit on.  Is it possible that your cells produce antisense transcripts 
of the particular gene you looked at?


Zhiyong Zhao



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