Anti-DIG fluorescein

Brendan Scott brendans at med.usyd.edu.au
Mon Aug 18 23:37:44 EST 1997


Konichiwa,

We have been trying to use anti-DIG fluorescein antibodies from Boehringer
Mannheim to detect DIG-labelled probes hybridised to RNA.  We haven't been
able to get it to work at all, in spite of fiddling with a variety of
conditions.  When we asked Boehringer Mannheim's Australian distributors
about our problems, they gave us a list of other people in Australia who
have been able to get it to work.  However, when we contacted six (6)
separate groups working with these antibodies on B-M's list, we found that
NONE of them could get it to work!

If there is anyone out there, in Australia or (especially) overseas who has
been able to get these antibodies to work?  What are we all doing wrong in
this country?  We would really appreciate being able to talk to someone
about this who has succeeded where we have failed.

Cheers,
Brendan Scott

P.S.  No attachments to any companies, B-M or otherwise.  We just want to
know what we are doing wrong.


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Brendan Scott BSc. PGDip(Hons. (Parasitology)) Qld

Institute of Dental Research            Department of Pathology
7th Floor                               5th Floor
United Dental Hospital                  Blackburn Building
cnr Elizabeth & Chalmers Sts            University of Sydney
Surry Hills NSW 2010                    Sydney NSW 2006
Australia
(02) 9282 0352                         (02) 9351 6143
Fax (02) 9282 0368                      Fax (02) 9351 3429
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