epitope tags for mammalian expression

Michael Gerdes mg690769 at bcm.tmc.edu
Tue Aug 19 10:54:48 EST 1997


Looking for some experiences on some of the epitope tags that people are
using in mammalian expression systems.  Merits/cons of HA, myc, etc.  I
need to localize a protein (suspected membrane protein) and would like to
detect by immunofluorescence and western of fractionated cells.  I would
like to clone the tag into the carboxy terminus.

Thanks,

Michael




More information about the Methods mailing list