Help???How to cip treatment with blunt-end vector???

[Mic Chaudoir]

In article <33F48865.F5F at liv.ac.uk>, User <dongliu at liv.ac.uk> wrote:

> Hi,
> Does someone know how to CIP treatment with blunt-end vector???
> many thanks,
> dong

Yes.. simply phosphorylate the PCR products.  Blunt-ended vectors can be
CIPed in the normal fashion.

-- 
Name:Mic Chaudoir    
E-mail:mic at nwu.edu
WWW:http://pubweb.acns.nwu.edu/~chaudoir/micpage.html
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" You don't have to swim faster than the shark, just faster than the guy next to you"- anonymous