How to clean DNA which may have glycosaminoglycans
Harry.Witchel at Bristol.ac.Uk
Tue Aug 19 17:34:54 EST 1997
Good day Molecular Charmers!
I have a problem with my DNA. Boy do I have a problem! My DNA
comes from a marine invertebrate which is hypocellular and filled with
gelatinous mesoglea which I'm told is made up of glycosaminoglycans or
hyaluronic acid. This thing is mostly connective tissue.
I have made the DNA, the animals are long gone, I have a limited
quantity. I have phenoled, chloroformed, ethanoled and isopropanoled
till blue in the face, but still, the stuff does not digest well with
restriction endonucleases. Even after running down the purified DNA
through Qiagen's genomic column, this DNA does not want to cut to
Are there any plant, cartilage, marine scientists out there who
have any suggestions on this sad fiasco.
YOur humble servant,
Harry.Witchel at bristol.ac.uk
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