How to clean DNA which may have glycosaminoglycans

Harry Witchel Harry.Witchel at Bristol.ac.Uk
Tue Aug 19 17:34:54 EST 1997


Good day Molecular Charmers!
	I have a problem with my DNA.  Boy do I have a problem!  My DNA 
comes from a marine invertebrate which is hypocellular and filled with 
gelatinous mesoglea which I'm told is made up of glycosaminoglycans or 
hyaluronic acid.  This thing is mostly connective tissue.
	I have made the DNA, the animals are long gone, I have a limited 
quantity.  I have phenoled, chloroformed, ethanoled and isopropanoled 
till blue in the face, but still, the stuff does not digest well with 
restriction endonucleases.  Even after running down the purified DNA 
through Qiagen's genomic column, this DNA does not want to cut to 
completion.
	Are there any plant, cartilage, marine scientists out there who 
have any suggestions on this sad fiasco.
	YOur humble servant,
	Harry

Harry.Witchel at bristol.ac.uk



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