hot-start PCR

Susan Hogarth sjhogart at unity.ncsu.edu
Wed Aug 20 15:54:36 EST 1997


Marc Champagne wrote:
 
> I've found that with most primer sets i can
> decrease my spurious bands by simply mixing my reactions at room temperature,
> instead of on ice, and by starting each PCR run with a 2 min at 94 extra-step.

Why does mixing at RT help?

-- 
Susan
"We have politics.  We just haven't offended each other with it yet.
Religion works just fine." --oTTo--
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