hot-start PCR

Susan Hogarth sjhogart at
Wed Aug 20 15:54:36 EST 1997

Marc Champagne wrote:
> I've found that with most primer sets i can
> decrease my spurious bands by simply mixing my reactions at room temperature,
> instead of on ice, and by starting each PCR run with a 2 min at 94 extra-step.

Why does mixing at RT help?

"We have politics.  We just haven't offended each other with it yet.
Religion works just fine." --oTTo--

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