Help???How to cip treatment with blunt-end vector???

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Aug 21 09:26:04 EST 1997


C.Rodemer (c.rodemer at dkfz-heidelberg.nospam.de) wrote:
> In article <33F4A548.5D90 at chugaibio.com>, dspinella at chugaibio.com,
> @chugaibio.com wrote:
> 
> > > Does someone know how to CIP treatment with blunt-end vector???
> > > many thanks,                                ^^^^^^^^^
>  
> I cut my PBluescript with EcoRI and didnt precipitate anything.I just
                            ^^^^^
> added buffer for CIP and some Units CIP .Then incubate at 37C.  

Seems I missed some recent development in restriction enzyme research.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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