Non-denaturing PAGE

[Richard Hastings]

Hi all,

anyone got a decent method for non-denaturing PAGE? I'm looking for a
complex of around 250kDa with a mind to cutting the complex and then
performing SDS-PAGE (ie. two dimensional PAGE).

Thanks for any help,

Richard.
--

Richard Hastings               TEL:+44 (0)115 9249924 ex. 44787
Dept. of Biochemistry          FAX:+44 (0)115 9422225
University of Nottingham       Email:mbxrh at mbn1.biochem.nottingham.ac.uk

Queens Medical Centre
Nottingham
NG7 2UH
U.K.