Help???How to cip treatment with blunt-end vector???
c.rodemer at dkfz-heidelberg.nospam.de
Fri Aug 22 04:43:05 EST 1997
In article <33FCB2D7.50FA at chugaibio.com>, dspinella at chugaibio.com,
> Well, you can get away with cutting corners once in a while, but sooner
> or later it comes back to bite you. No doubt your own forthcoming recipe
> compilation will be far superior.
Its the old problem : "We've done it 20years like that, so its good." Dont
you think there's sometimes a improvement in the protocols necessary ?
> You're a cloning stud! However, since the original poster didn't say
> what restriction enzyme he was using for blunting, I thought it best to
> send him the "long" procedure since Cip isn't always fully active in all
> restriction enzyme buffers.
Yes, its ok, it wasnt meant as a criticism or insulting you.
> Way to go, ace! Looks like next year's Nobel is a lock for ya.
Don't be ironic. Ive got problems enough, so let me celebrate a little
bit of success...
To send me Email, remove .nospam from my address.
More information about the Methods