looking for erythromycin sensitive E.coli

H. Yim hyim at u.washington.edu
Mon Aug 25 12:34:19 EST 1997

The strain MC1061 has worked well for us at ~300-400 ug/ml of
erythromycin.  If you have the wild type gene, you might have to
pre-induce erm resistance with ~0.1 to 1 ug/ml.  You will end up with a
haze of background bacteria with larger colonies that have your plasmid.
Good luck,

Harry Yim Ph.D.
Dept of Infectious Diseases
Children's Hospital and Medical Center
Seattle WA 98105

hyim at chmc.org  
hyim at u.washington.edu
(206) 528-2767
fax (206) 527-3890

On Fri, 22 Aug 1997, zhuqing li wrote:

> Hi
> Can anybody help me to find out E.coli strains that are sensitive to
> erythromycin so that they can be used as host to clone a gene which
> encodes erythromycin resistance product?  For my knowledge, the MIC of
> Em against E.coli is >120ug/ml.  
> Thanks a lot in advance!
> Zhuqing Li
> Dept. Microbiol. & Immunology
> Med. College of Ohio
> Toledo, OH 43699
> E-mail: ZLI at opus.mco.edu

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