End-polishing and ligation

zzzz zxz4 at PSU.EDU
Mon Aug 25 09:22:52 EST 1997


My two sents:

(1) 130ng DNA in 10ul sounds too much.
(2) Use TAE in stead of TBE buffer for gel purification. 
(3) I use NEB Buffer 2 for ligation; it works fine.



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