End-polishing and ligation

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Mon Aug 25 09:51:50 EST 1997


In article <34017EFB.29EC at hkucc.hku.hk>, ywlee at hkucc.hku.hk wrote:

<snip>
Alan 

Have you tried blunting the Acc I (Klenow or T4) first, followed by
clean-up (phenol-chlor/EtOH precip), then doing the ligation as a separate
reaction??

Cheers

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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