Supercoiled DNA/transfection

Vladimir Svetlov svetlov at oncology.wisc.edu
Tue Aug 26 14:36:02 EST 1997


In article <33F84BF9.1DB0 at box-d.nih.gov>, "Darren A. Natale"
<dnatale at box-d.nih.gov> wrote:

riginally REMOVED negative supercoils, taking it away will again
> ADD 
> negative supercoils.
> 
> D. Natale
> dnatale at box-d.nih.gov

Ya, guys and gals, are too sophisticated for your own good - kinda hard to
be a-keeping track of them supercoils <G>. The easy way to "fix" the nicked
DNA is to reinoculate (yes, and retransform if needed) the e. coli and
isolate the DNA, right way this time, so it is not nicked. Good luck with
transfection with DNA ligated in presence of EtBr. 
Yours,
Pointless peasant Baldrick.



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