DAPI solubility in PBS

Annette C. Hollmann ah690549 at bcm.tmc.edu
Tue Aug 26 08:59:48 EST 1997


In article <v01550100b02765970ec7@[142.20.39.36]> jlight at SICKKIDS.ON.CA (Jeff Lightfoot) writes:
>I'm staining COS7 cells with DAPI and want to stain in PBS.  I have DAPI
>from SIGMA, but they recommended solubilizing it in water because it's not
>soluble in PBS.  I've used DAPI before from another source and did the
>staining in PBS.  I suppose that DAPI powder has to be solubilized in
>something other than water so that it will solubilize in PBS, but I don't
>know what that chemical would be.  Could someone let me know how to
>solubilize DAPI in PBS, and which company they purchase it from?  Thanks in
>advance.

I got some DAPI from Molecular probes.
I have a 2 mg/mL stock solution in dH2O, of which I added 1 uL to 99 uL of
PBS for use in staining (final concentration 20 ug/mL).
I can probably use a lot less than that next time - the staining was
*incredibly* bright.

Annette



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