Help???How to cip treatment with blunt-end vector???

bipin k. dalmia dalmiabk at phibred.com
Tue Aug 26 11:15:49 EST 1997


On 26 Aug 1997 08:11:23 -0700, dspinella at chugaibio.com (Dom Spinella)
wrote:
>This thread seems absurdly long given the triviality of the original
                                ^^^^^^^^^^
the most number of posts were from you Dom.

>question, i.e. how to Cip treat a blunt-cut plasmid. My original
>response to the posting was adapted virtually unchanged from Sambrook et
>al. and yet to my surprise, seems to have provoked all kinds of
>disagreement (some of it rather strident in tone).  In the hope of
                                                   ^^^^^^^^^^^
and one of your posts was the most strident of 'em all.

not to beat a dead horse, but had to get the record straight. these
posts are archived you know :)

bip 



Bipin K. Dalmia, Ph.D.
Protein Expression and Purification
Pioneer Hi-Bred International, Inc.
Johnston, Iowa 50131

dalmiabk at phibred.com



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