Help with orthophosphate labeling!

[NJH at mail.nox.ac.uk]

. Still, I got a lot of background and a very weak band. 
>Is it worth trying a longer incubation with 32P, or starting with more
>cells? How can I remove the background?
>Any idea would be very helpful!

When you say background, do you mean other labelled proteins or just "hot 
stuff" (like a smear) in your lanes? For the smear I found that 
electroblotting onto a membrane and exposing the membrane reduces the 
background.

Nigel