rep. Bsa BI

rabc010 at S1.CXWMS.AC.UK rabc010 at S1.CXWMS.AC.UK
Wed Aug 27 06:24:46 EST 1997


You should checkyour E.coli strain from which oyu have isolated
plasmid. Your plasmid may not be cut if it was isolated from the
bacterial strain expressing Dam methylases. Because this can
methylase your site.
I hope that is useful.

narendra
nkaushik at hgmp.mrc.ac.uk



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