What orange or yellow dye to use in DNA electrophoresis?
CANTOLUPO at GENVEC.COM
Wed Aug 27 12:11:59 EST 1997
Orange-G is the one to go with. It runs at about 100bp on a 1% gel (a
little faster than bromo blue). Make it up like a bromo blue dye but
use twice as much orange-g. Be carefull with acridine orange; it's just
like ethidium bromide and has the same intercolation properties (i.e.
it's a big nasty).
Does anyone know of any other colors to run on a gel, mainly between
xylene cyanol and bromo blue? I'm trying to make a spectrum of dyes
just for fun! <the boss doesn't know about this!!!!>
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