What orange or yellow dye to use in DNA electrophoresis?

Lou Cantolupo CANTOLUPO at GENVEC.COM
Wed Aug 27 12:11:59 EST 1997


Orange-G is the one to go with.  It runs at about 100bp on a 1% gel (a
little faster than bromo blue).  Make it up like a bromo blue dye but
use twice as much orange-g.  Be carefull with acridine orange; it's just
like ethidium bromide and has the same intercolation properties (i.e.
it's a big nasty).  

Does anyone know of any other colors to run on a gel, mainly between
xylene cyanol and bromo blue?  I'm trying to make a spectrum of dyes
just for fun!  <the boss doesn't know about this!!!!>

Lou Cantolupo
GenVec, Inc.
Dept. Molecular Virology
12111 Parklawn Drive
Rockville, MD  20852
(301) 816-0396 x217




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