What orange or yellow dye to use in DNA electrophoresis?

David Micklem drm21 at mole.bio.cam.ac.uk
Wed Aug 27 12:43:59 EST 1997


In article <5u1jtu$qlo at maze.dpo.uab.edu>, levy at uab.edu (David N. Levy) wrote:

>I cannot remember which yellow or orange dyes can be used to make a
>loading buffer for agarose electrophoresis. I thought it was acridine
>orange, but my acridine orange from Sigma fluoresces a lot.  But they
>list it in the nucleic analysis section of their catalog.  What gives? 
>Thanks.
>
>
>
>David N. Levy
>University of Alabama at Birmingham
>Birmingham, AL 35294-0007
>
>levy at uab.edu

Acridine orange?  Ugh! 

Try Orange G - works a treat.

David

-- 
D.R.Micklem,                                Time flies like an arrow... 
Wellcome/CRC Institute,            Fruit flies like a banana.       
Cambridge CB2 1QR, UK               Email:drm21 at mole.bio.cam.ac.uk
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