What orange or yellow dye to use in DNA electrophoresis?

bqamar at paknet1.ptc.pk bqamar at paknet1.ptc.pk
Wed Aug 27 22:51:28 EST 1997

In article <5u1jtu$qlo at maze.dpo.uab.edu>,
  levy at uab.edu (David N. Levy) wrote:
> I cannot remember which yellow or orange dyes can be used to make a
> loading buffer for agarose electrophoresis. I thought it was acridine
> orange, but my acridine orange from Sigma fluoresces a lot.  But they
> list it in the nucleic analysis section of their catalog.  What gives?
> Thanks.
> David N. Levy
> University of Alabama at Birmingham
> Birmingham, AL 35294-0007
> levy at uab.edu


I routinely run "Orange G" on agarose gels. On a 2% gel it runs with the
dye front so is quite useful.  I think we bought it from Sigma Chemicals,
and at least under UV it does not fluoresce.

Raheel Qamar

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