What orange or yellow dye to use in DNA electrophoresis?
michelle at MOLECULE.BIO.UTS.EDU.AU
Wed Aug 27 17:34:26 EST 1997
I make a tri colour dye using bromphenol blue, xylene cyanol, and orange
G. The orange G runs about 50bp on the gel, and I just added the same
quantity as for the other dyes. Our haematology/cytology lab had it, so
that would be a good place to start looking. I think I got the recipe
from the Promega catalogue, who sell a pre made tri colour dye.
Molecular Parasitology Unit Ph: (02) 9514 4043
University of Technology Fax:(02) 9514 4003
Westbourne St Gore Hill, NSW 2065 michelle.gleeson at uts.edu.au
When you get to the end of your rope, tie a knot and hang on - FDR
On 27 Aug 1997, David N. Levy wrote:
> I cannot remember which yellow or orange dyes can be used to make a
> loading buffer for agarose electrophoresis. I thought it was acridine
> orange, but my acridine orange from Sigma fluoresces a lot. But they
> list it in the nucleic analysis section of their catalog. What gives?
> David N. Levy
> University of Alabama at Birmingham
> Birmingham, AL 35294-0007
> levy at uab.edu
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