in situ hy help

Tim Smart seb at alto.com
Thu Aug 28 09:18:56 EST 1997


On 26 Aug 1997 21:18:36 -0600, zinc  said:

>call me a curmudgeon if you want but all these kits just make me
>gag. i still purify my DNA using alkaline lysis and CsCl
>gradients. with the new ultracentrifuges this can easily be completely
>in less than one day.

>at least this way i get a ton of DNA and don't have to worry about any 
>contaminants.

>-pjf

yeah, and CsCL and EtBr are much better for the enviroment too, and running 
an ultra centrifuge doesn't cost as much energy as well.

Tim








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