in situ hy help

Tim Smart seb at
Thu Aug 28 09:18:56 EST 1997

On 26 Aug 1997 21:18:36 -0600, zinc  said:

>call me a curmudgeon if you want but all these kits just make me
>gag. i still purify my DNA using alkaline lysis and CsCl
>gradients. with the new ultracentrifuges this can easily be completely
>in less than one day.

>at least this way i get a ton of DNA and don't have to worry about any 


yeah, and CsCL and EtBr are much better for the enviroment too, and running 
an ultra centrifuge doesn't cost as much energy as well.


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