5' end labeling

cfb7 at psu.edu cfb7 at psu.edu
Thu Aug 28 16:10:26 EST 1997


I want to do a Southern hybridization using an oligo probe that is very
short (25 bases), yet very specific.  How much would I need to end
label relative to the amount I usually random-primed label (50 ng of
700 bp)?  The Promega protocol book says to use <10 pmol, but they must
mean if the probe is longer?  Thanks, C- 

Christie Blackman          cfb7 at psu.edu
Department of Biochemistry and Molecular Biology
Penn State University
University Park, PA



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