testing Cre recombination

Robert M. Mihalek mihalek at smtp.anes.upmc.edu
Tue Aug 12 15:25:59 EST 1997


we would like to test some constructs containing loxP sites just to
make sure they work prior to transfection. Does anyone know of a source
of purified Cre protein for an in vitro assay? Or, perhaps we could use
a bacterial strain that expresses Cre protein. I read that BS591 is a
recA strain that has a lambda cre prophage. However, I can't find a
source for this bacterial strain. Any suggestions?

Thanks in advance.

Bob Mihalek
University of Pittsburgh


 



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