GFP acceptor dye for FRET

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Aug 28 13:27:30 EST 1997


Nico Dantuma (nico.dantuma at mtc.ki.se) wrote:
> I am looking for suggestions on what dyes can be used for establishing FRET
> (fluorescence resonance energy transfer) with a GFP that has a wild type
> emission spectrum (510 nm).  The dye will be used for protein labeling.  I
> am especially interested in people who have experience with using GFP in
> FRET experiments.

Could you forward the replies you get? I spoke with Werner (?) Almers from
the MPI in Heidelberg and he told me that he thinks FRET with GFP is
impossible.

Thanks in advance,

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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