Help! Separating 2 PCR products.

TODD GORSUCH RISS at onaustralia.com.au
Fri Aug 29 06:26:56 EST 1997


Dharsha,

I had a similar problem with a clinical PCR for Legionella. The problem was
overcome by reducing the concentration of Taq. 

Another suggestion, if all else fails, is to use an enzyme cut unique to
one of the amplified sequences after amplification. This will seperate your
bands on a gel.

Hope I could help.

Todd
todd at micr.crg.cs.nsw.gov.au





More information about the Methods mailing list