Genomic DNA extraction

Rick Bright rbright at emory.edu
Fri Aug 29 19:47:38 EST 1997


I have personally modified several previously published extractions protocols
and developed a single protocol that extracts genomic DNA from whole blood in
under 2 hours.  It is non-toxic (no phenol or chloroform) and produces a very
pure product without using any further purifications steps.  IT also yeilds
approx 100ug of DNA from 5 ml of whole blood.  The only problem is that I have
not published it yet, and have since changed labs and am without the guidance
to publish the protocol.  I would be happy to share it, but would also
appreciate assistance in getting it into publication.

Email me if you want further information.

Rick Bright
rbright at emory.edu

Laurent GALIO wrote:

> I would like to use an extraction protocole for genomic DNA of human cells
> without organic extraction (no phenol-no chloroform).
>
> I tried one method which says to digest the cells with K proteinase
> (100µg/ml), during 1 hour at 60°C and then during 16 hours at 37°C.
> After that I added Na Acetate (100mM final concentration) and two volumes
> of ethanol. I began to see the DNA and when I mixed gently: it
> dissapeared...
>
> Please if you have used, with success, a protocole to extract genomic DNA
> without Phenol/chloroform, send it to me with the exact conditions.
>
> Thanks...
>
> *********************************************************************
> Laurent GALIO
> Centre National de Séquençage
> Généthon
> 1, rue de l'Internationale
> 91 000 Evry, France
> *********************************************************************






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