de-glycosylation

[Roger Murphy]

Hi fellow protein chemists!

Does anyone have a quick, simple recipe for removing N- and/or O-linked 
glycans from monoclonal antibodies?  We want to determine the protein MW with 
and without post-translational modification of the glycan chain.

Thanks,

Roger Murphy



Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084
Australia.

Tel  61-3-94965463
Fax  61-3-94965436
Email murphy_r at licre.ludwig.edu.au