NdeI versus NcoI... which is best?

Richard J. Dudley rdudley at nrc.uab.edu
Mon Dec 1 10:28:15 EST 1997


You also have to look at the recognition sequences for the wo enzymes. 
NcoI has the ATG in the middle of the site (C!CATGG), whereas NdeI has
the ATG at the end (CA!TATG).  NdeI is a very poor cutting enzyme;
according to NEB, it has a half life of 15 min at 37 C.  From persal
experience with NdeI, it takes a lot of patience to get good
religattable digests with NdeI.

rich

--- --- --- -- -- -- --- --- ---
* Experience is something you don't get until just after you need it. *

Richard J. Dudley (rdudley at nrc.uab.edu)                            
Department of Neurobiology                    
University of Alabama School of Medicine       
http://www.nrc.uab.edu/



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