Seeking info on bacteria strain CJ236

[Ralf Oltmanns]

Kirstin Therese Bopp wrote:

> I am trying to isolate single stranded DNA for use in site directed
> mutagenisis.  I am using the bacteria strain CJ236 and cannot seem to
> prevent it from lysing and contaminating my prep with genomic DNA.  Does
> anybody have any information on the growth conditions of this strain or
> suggestions on how to prevent it from lysing.
>
> Thanks,
> Kirstin

 Hi Kirstin,

you might have a look at the Website of the DSMZ (Germany Collection of
Microorganisms), especially page
http://www.gbf-braunschweig.de/DSMZ/strains/no009851.htm

You could also give the a call (see:
http://www.gbf-braunschweig.de/DSMZ/dsmzadr.htm) or ask the by email
(dsmz at gbf.de).  The should know how to cultivate their own critters.

Bye
Ralf


--
### We, the Willing,
### led by the Ignorant,
### do the Impossible for the Ungrateful