Liquid nitrogen and glassware

brett brett at BORCIM.WUSTL.EDU
Wed Dec 3 09:52:22 EST 1997


>Michael Allen wrote:
>
>> Hello everyone
>>
>> I would like to grind single Arabidopsis leaves in a conical glass
>> tube and
>> pestle set for the extraction of total RNA.  Will it stand up to
>> liquid
>> nitrogen, or am I just looking to break expensive glassware?
>
>That's something I'd like to know, too. I would much appreciate any
>advice on this by mail.
>
>Thanx.
>Ralf
>

Bring your glassware down to temp gradually. You might try -20C -> -80C ->
liquid propane -> liquid N2. Also, don't use a pounding motion during the
homogenization.
Brett Lindenbach
brett at borcim.wustl.edu

Dept Molecular Microbiology
Washington University School of Medicine
Box 8230
660 S. Euclid Ave
St Louis, MO 63110




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