cloning small linker fragments

Clemens Kittinger clemens.kittinger at kfunigraz.ac.at
Wed Dec 3 08:46:02 EST 1997


I´ve a small linker fragment ( 51 bp ) I want to clone in a 4,2 kb large
vector. Sorrowly I´ve to clone it Xba I - Xba I. I´ve played a lot on
the ratio vector vs insert, but I didn´t get clones until now.

If you have experience in cloning such small fragments, please answer
me.

Gratefully 
Clemens Kittinger
Inst. f. Mikrobiolgie
KF Uni Graz
Austria



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