Staining on DNA on nylon or nitrocellulose membranes

Bo Pontoppidan bo.pontoppidan at cellfo.slu.se
Wed Dec 3 17:01:58 EST 1997


Reinhard M G Eckloff wrote:
> 
> HI
> I am interested in staining DNA that has been transferred to a
> nitrocellulose or nylon membrane.
> I know this can be done by an antibody labelling. But I am looking for a
> stain like Ethidiumbromide or China ink. Does anybody have experience
> with this?
RNA on nylon membranes (NOT nitrocellulose) can be stained with 0.03%
methylene blue and 0.3 M sodium acetate pH 5.2 for half a minute
followed by a 2 minute rinse with water. 

Bo
--
Bo Pontoppidan                                  Phone: (+46) 018 67 33
24
Uppsala Genetic Center                            Fax: (+46) 018 67 32
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Dept. of Cell Research                     Home Phone: (+46) 018 69 28
38
Swedish University of Agricultural Sciences, Box 7055,  
S-750 07 Uppsala, Sweden             E-mail:
bo.pontoppidan at cellfo.slu.se



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