cloning small linker fragments

Chulho Kang kang at msvax.mssm.edu
Thu Dec 4 02:19:22 EST 1997


Clemens Kittinger wrote:
> 
> I´ve a small linker fragment ( 51 bp ) I want to clone in a 4,2 kb large
> vector. Sorrowly I´ve to clone it Xba I - Xba I. I´ve played a lot on
> the ratio vector vs insert, but I didn´t get clones until now.
> 
> If you have experience in cloning such small fragments, please answer
> me.
> 
> Gratefully
> Clemens Kittinger
> Inst. f. Mikrobiolgie
> KF Uni Graz
> Austria

 Dear Clemens;

Please read the following article. I will work loke a charm.



Biotechniques 1993 Oct;15(4):659-662 

One-step insertion of oligonucleotide linkers or adapters
to DNA using unphosphorylated oligonucleotides.

Kang C, Inouye M



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