3'-specificity of Taq

Dr. V. Blaschke vblasch at gwdg.de
Thu Dec 4 05:31:43 EST 1997


Dear fellow researchers,

I think it is well established that Taq processivity in PCR depends on
tight 3'-binding of primers. Does anyone know exactly how many
mismatches at the 3'-end are necessary to completely abolish Taq
activity, i.e. would a 24-mer primer with a perfect overall match except
for a total mismatch of the three last 3'-bases work in PCR or not?

Any suggestions?

Thanks,

   Volker



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