how to separate vector dna from chromosomal dna

Christian Fetzer cfetzer at uni-wh.de
Thu Dec 4 12:39:48 EST 1997


hi folks...

help needed !!!
we are looking for a possibility to separate vector dna from
chromosomal dna in eukaryots. we have tried the so-called HIRT-Method
so far, but it contained still chromosomal dna, as we could see by
agarose-gel-elektrophoresis. any solutions for our problem are very
welcome.

c. fetzer & c. piechaczek, institute of cell biology, university of
witten herdecke, germany



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