proteinase K storage question

Chulho Kang kang at
Sun Dec 7 03:07:38 EST 1997

Farida Safadi-Chamberlain wrote:
> Yes i dissolve Proteinase K in 50mM tris pH 8.0 and 50% glycerol, I store
> at -20 C. It works fine
Hi There;

Yes, I totally agree. I am also dissolve P.K. in 50% glycerol solution 
(in water) and store in -20 C. It works fine as far as you do not take
the bottle or tube out of the freezer too often (Don't forget P.K. is a
self digesting enzyme like many other proteinases). Alternative method
is dissolving P.K in water and lyophilizing it after you dispense it
into an appropriate tube. 

Chulho Kang
Kang at

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