proteinase K storage question

[Chulho Kang]

Farida Safadi-Chamberlain wrote:
> 
> Yes i dissolve Proteinase K in 50mM tris pH 8.0 and 50% glycerol, I store
> at -20 C. It works fine
> 
Hi There;

Yes, I totally agree. I am also dissolve P.K. in 50% glycerol solution 
(in water) and store in -20 C. It works fine as far as you do not take
the bottle or tube out of the freezer too often (Don't forget P.K. is a
self digesting enzyme like many other proteinases). Alternative method
is dissolving P.K in water and lyophilizing it after you dispense it
into an appropriate tube. 


Chulho Kang
Kang at hifax.com
http://hifax.com