colony PCR for E.coli genomic sequences

Richard Koepsel rrk1+ at pitt.edu
Mon Dec 8 09:52:03 EST 1997


Netters,
Does anyone have experience with detecting E.coli genomic sequences
using colony PCR?  We find ourselves in the position of having to screen
for a deletion (which we are trying to construct) that has no selectible
phenotype.  We would like to avoid colony screens if possible.  We can
amplify the target from purified chromosomal DNA and we can do colony
PCR of the gene cloned in a pUC plasmid.  Any suggestions?
Thanks,
Rick Koepsel



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