Any experience with Hind3 on a primer?

Kiley R. Prilliman kileyrp at
Mon Dec 8 21:21:48 EST 1997

In article <348AC413.727C8CC8 at>,
Harry.Witchel at wrote:

> Hi folks --
>     Anyone got any experience using Hind3 on a PCR primer for
> directional cloning?  I would very much like to use Hind3, but according
> to the appendix of the New England Biolabs catalogue ("Cleavage close to
> the end of DNA fragments") Hind3 is quite inefficient cutting near the
> ends of DNA (eg at the 5' end of a PCR product).
>     Thanks much,
>         Harry

I use HindIII in some of my primers for directional cloning, and we did
the same thing: extended by 5-6 bases or great.

Kiley R. Prilliman
Department of Microbiology & Immunology
University of Oklahoma Health Sciences Center

phone: 405-271-1203
  fax: 405-271-3117

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