Any experience with Hind3 on a primer?

Kiley R. Prilliman kileyrp at geocities.com
Mon Dec 8 21:21:48 EST 1997


In article <348AC413.727C8CC8 at Bristol.ac.Uk>,
Harry.Witchel at Bristol.ac.uk wrote:

> Hi folks --
>     Anyone got any experience using Hind3 on a PCR primer for
> directional cloning?  I would very much like to use Hind3, but according
> to the appendix of the New England Biolabs catalogue ("Cleavage close to
> the end of DNA fragments") Hind3 is quite inefficient cutting near the
> ends of DNA (eg at the 5' end of a PCR product).
>     Thanks much,
>         Harry

I use HindIII in some of my primers for directional cloning, and we did
the same thing: extended by 5-6 bases or so...works great.

Kiley R. Prilliman
Department of Microbiology & Immunology
University of Oklahoma Health Sciences Center

phone: 405-271-1203
  fax: 405-271-3117



More information about the Methods mailing list