Library screening w/ oligos?

z z-suldan at ski.mskcc.no.spam.org
Mon Dec 8 12:04:20 EST 1997


In article <881603359.11947 at dejanews.com>, bbeitzel at biomail.ucsd.edu wrote:

> I was wondering if anyone had experience screening libraries with oligos.
> Specifically, I have a 4-fold degenerate 20 mer that I would like to use
> to screen a lambda library.  Is this possible?  Anyone have any protocols
> or know of any good papers on this?
> 
> Thanks for any info,
> 
> Brett Beitzel
> bbeitzel at biomail.ucsd.edu
> Infectious Disease Lab
> The Salk Institute
> 
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Both Maniatis and the red book (Current protocols) have sections on this.


Good luck,
Z



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