Immobilizing Trypsin

Peter Wang plw at
Tue Dec 9 13:12:36 EST 1997

Yoram Gerchman wrote:
> greetings netters. Dose anyone know of a way to immobilize Trypsin to a
> resin or something so it will a) wont cut itself and b) will be easily
> removed from the reaction ? Maybe a commercial product ?

Dear Yoram:
Pierce (and probably others) sells immobilized trypsin and immobilized 
soybean trypsin inhibitor; the latter allows you to digest with soluble 
trypsin and then remove it.

Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)

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