Immobilizing Trypsin

Peter Wang plw at mrc-lmb.cam.ac.uk
Tue Dec 9 13:12:36 EST 1997


Yoram Gerchman wrote:
> greetings netters. Dose anyone know of a way to immobilize Trypsin to a
> resin or something so it will a) wont cut itself and b) will be easily
> removed from the reaction ? Maybe a commercial product ?

Dear Yoram:
Pierce (and probably others) sells immobilized trypsin and immobilized 
soybean trypsin inhibitor; the latter allows you to digest with soluble 
trypsin and then remove it.

---------------------------------------------------------
Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
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