Cloning of large retroviral vectors

Quicksilver beckerr at vzdmzd.zdv.uni-mainz.de
Tue Dec 9 06:52:25 EST 1997


Hi newsgroupers,

we have tremendeous problems in cloning a retroviral vector (about 10 
kb) in E.coli C600 or HB101. Our bacteria grow very good in LB-amp but 
the plasmid yield is extremely low. We tried Qiagens as well as 
Machery and Nagels midi and maxi plasmid prep kits.

Any suggestion is appreciated.

Thanks a lot

Roger



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