2.:Re: PCR Screening of transformants?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Tue Dec 9 13:22:17 EST 1997


Works well with Taq (-equivalent) from PAN systems and Appligene, at 
least for my system. I take a .5ul sample of a miniprep pellet 
(dissolved in 200ul TE with RNAse added, what I start my minipreps 
from) directly in a vol of 10ul PCR mix, and start the PCR then 
without special precautions. My EC strain is XL 1-blue.

Cheers, Wolfi
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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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