Preparing electrocompetent yeast cells

z z-suldan at ski.mskcc.no.spam.org
Thu Dec 11 07:38:37 EST 1997


In article <348eefcf.1706734 at news.nih.gov>, cxs419 at psu.edu (John Sperati) wrote:

> Hi,
> 
> Does anybody have a protocol (or can point me in a direction) for
> preparing electrocompetent S. cerevesiae that permits freezing of the
> cells for long term storage?  Basically, I'd like to make a large
> batch of electrocompetent cells and then store them at -80C, as is
> possible with E. coli.  Can this be done?
> 
> Thank you.
> 
> --John Sperati

Current Protocols (The red book) has a very straight forward (and
relatively easy) protocol. Works well and they freeze well.

Good luck,
Z



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