Preparing electrocompetent yeast cells

z z-suldan at
Thu Dec 11 07:38:37 EST 1997

In article <348eefcf.1706734 at>, cxs419 at (John Sperati) wrote:

> Hi,
> Does anybody have a protocol (or can point me in a direction) for
> preparing electrocompetent S. cerevesiae that permits freezing of the
> cells for long term storage?  Basically, I'd like to make a large
> batch of electrocompetent cells and then store them at -80C, as is
> possible with E. coli.  Can this be done?
> Thank you.
> --John Sperati

Current Protocols (The red book) has a very straight forward (and
relatively easy) protocol. Works well and they freeze well.

Good luck,

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