non-reducing SDS-PAGE

Alex Chang lpss at unixg.ubc.ca
Thu Dec 11 16:38:06 EST 1997


I'd like to know anyone has tried maleimide 1mM, in addition to take away
the reducing reagents, 2-ME and DTT in the loading buffer, to make the
bands sharper in the non-reducing SDS-PAGE. 


Alex
Pathology
University of British Columbia
alex at brc.ubc.ca



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