Bermuda Quality Sequences
wgschech at med.uni-tuebingen.de
Thu Dec 11 05:41:40 EST 1997
Hi Randy! Bermuda Quality Sequences are determined using the
1) ask your colleague to figure out a number between 100 and 773.
This number is n, the length of your sequence. You also may ask the
whole staff of your institute and calculate the arithmetic average
value of all numbers given.
2) take a tetraedic dice, write the letters A,C,G and T on it (if you
don't have such a high sophisticated device, you instead can use a
common cubic dice and label one plane with N and the other with "roll
again". But note that cubic dice sequencing is not as good as
tetraedic dice seqencing, since the cubic dice will not resolve one
of five nucleotides!
3) roll the dice n-times and write down the letters carefully
4) in times of computational chemistry, the dice may be simulated by
computer algorithms calculating the physical behavior of a dice (but
not by (pseudo)random number generators, they are not accurate
5) proof read the sequence by performing this procedure three
times (that's good laboratory practice). Doing the experiment by two
independend labs is highly recommended.
6) Sequence data determined by this method currently is accepted by
a) The Journal of Irreproducible Results (published in Bermuda)
b) The Gnomic Database
c) The April 1st issues of Science, Nature, JBC and PNAS (yet to be
decided by the editorial boards)
If you need a reference:
AU: Eulenspiegel, T and Marsupilami, W.S.
TI: A novel, cheap and funny way of determining genetic sequences
JO: Doing Research in Times of Limited Funding, Vol 1 (Dec 1997),
> What is meant by "Bermuda Quality Sequences"? What is the level of
> quality? What is the origin of the term? What data bases or
> organizations recognize this level of quality?
> Randy Morse
> rhmorse at genbiosys.com
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